Description: Participants will learn how to use DESeq2 to identify differentially expressed genes and become familiar with design matrices. The session will also introduce concepts essential for working with real-world data, such as incorporating covariates and interaction terms into models. Additionally, participants will learn how to apply Over-Representation analysis (ORA) and Gene Set Enrichment Analysis (GSEA) for uncovering biologically meaningful patterns. Hands-on examples and exercises will help reinforce concepts and guide participants through a typical RNA-seq workflow.

Pre-requisites:

1. Experience with R (RStudio installed)
2. DESeq2 package

NOTE: Please be advised that persons funded by D2R are given priority for in-person attendance. If you are not funded by D2R, please register for the online workshop, and mark whether you would prefer to be moved to the in-person workshop. We will adjust attendance based on availability the week before the workshop.

Instructor: Ariel Madrigal Aguirre – PhD Candidate, Dept. Human Genetics, McGill University

Facilitator: Zahra Tavakol – PhD Student, Quantitative Life Sciences Program, McGill University

Location: HYBRID. Online via Zoom, or in-person at the Education Building, Room 133

IMPORTANT NOTE: for those attending the workshop online, you must log-into your McGill Zoom account. Otherwise, the link will not work. You can do so on the browser here: https://mcgill.zoom.us/ . For more information on McGill Zoom, please visit this website: https://www.mcgill.ca/tls/students/learning-resources/use-technology/learning-zoom